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KMID : 0855520090220040255
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Korean Journal of Physical Anthropology
2009 Volume.22 No. 4 p.255 ~ p.268
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A Kinship Analysis of Ancient Human Bones and Teeth from Mongolia
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Munkhtsetseg Bazarragchaa
Kim Ki-Jeong
Kim Jae-Hyun
Gavaachimed Lkhagvasuren
Park Ae-Ja
Lee Kwang-Ho
Kim Dae-Jin
Chung Yoon-Hee
Kim Sung-Su
Lee Won-Bok
Kim Kyung-Yong
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Abstract
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The kinship was analyzed genetically on the three 2000 year old ancient human bones and teeth excavated in Mongolia. The samples were processed in a clean room to prevent the contamination from modern human DNA. The DNA extraction and purification was done with ion-exchange column kit (Qiagen G-tip 20G, USA). The PCR was done with purified DNAs from ancient human bones for paternal Y-SNP haplogroup, maternal mtDNA haplogroup, and autosomal short tandem repeats (STR). Two samples belonged to the maternal D major haplogroup, which is one of the most frequent types in the present North East Asia. One of them, showing male genotype, belonged to the paternal C major haplogroup, which is also one of the most frequent types in the present North East Asia. The remaining one belonged to the paternal R major haplogroup, frequent in the present Europe, and the maternal U haplogroup, frequent in the present Europe and East Mediterranean. The repeated results were consistent in the autosomal STR PCR. The STR data were analyzed with DNA-VIEW program (http://www.dna-view.com), which showed no close kinship among the three ancient humans. Our method was successful in the analyzing kinship among ancient human bones, which has been possible in few restricted laboratories in the World. Authors anticipate that many researchers could do their research in a better way to get the genetic information from ancient human bones.
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KEYWORD
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Ancient human bones, Teeth, STR, DNA, PCR, Kinship
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